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. 2019 Mar 15;23(5):3563–3571. doi: 10.1111/jcmm.14255

Figure 4.

Figure 4

Effect of naftopidil on the migration and responsiveness to TGF‐β stimulation in human lung fibroblast lines. A, Representative images of scratch wound‐healing assay at 0 and 12 h. The left two columns show cells treated with dimethyl sulfoxide (DMSO) alone. The right two columns show cells treated with 40 µmol/L naftopidil. The top, middle and bottom rows displayed WI‐38, TIG‐1‐20 and LL97A cells, respectively. B‐C, Ratios of mRNA expressions of α1 chain of type IV collagen (B, COL4A1) and α‐smooth muscle actin (C, α‐SMA) to GAPDH in the same sample were analysed via real‐time quantitative reverse transcription‐polymerase chain reaction and expressed relative to results for cells cultured without TGF‐β1 (=1.0) in the same experiments. Black columns represented ratios of mRNA expressions in the cells treated with 5 ng/mL TGF‐β and DMSO. White columns represented ratios of mRNA expressions in the cells treated with 5 ng/mL TGF‐β and 40 µmol/L naftopidil. Data are means ± SEM from four experiments