Figure 3.

Knockdown tetraspanin 1 (TSPAN1) promoted cell migration and transforming growth factor‐β1 (TGF‐β₁)‐induced epithelial‐to‐mesenchymal transition‐related proteins in alveolar epithelial cells. A, Wound healing assay following silencing of TSPAN1 in A549 cells. B, Quantification results of wound healing assay. Bar chart represents the ratio of every group to negative control (NC) groups. The data are expressed as the means ± SEM (n = 3), with results representative of three independent experiments. (*P < 0.05 vs NC). C, Transwell migration analysis by silencing TSPAN1 in A549 cells with or without TGF‐β₁ treatment (5 ng/mL, 16 h). D, Quantification of transwell migration analysis. The bar chart represents the numbers of migrating cells. The data are expressed as the means ± SEM (n = 3), with results representative of three independent experiments. (**P < 0.01 vs NC). (E,G) Western blot analysis of various proteins by silencing TSPAN1 for 24 h in A549 and alveolar epithelial type II cells with or without TGF‐β₁ treatment. F, Quantification of the results in panel (E), and (H) quantification of the results in panel (G). The data are expressed as the means ± SEM (n = 3), with results representative of three independent experiments. (*P < 0.05, **P < 0.01, ***P < 0.001 vs NC 0 h; ^# P < 0.05, ^## P < 0.01, ^### P < 0.001 vs NC 12 h; ΔP < 0.05, ΔΔP < 0.01, ΔΔΔP < 0.001 vs NC 24 h)