Table 3.

Quantitative PCR analysis of cytokines profile and TLR2 and TLR4 mRNA expression in mixed hippocampal cultures stimulated with purified LPS of serotypes a, b, or c of Aa.

IL-1β	Fold change	IL-4	Fold change	IL-6	Fold change	IL-10	Fold change
Serotype a	3.82 ± 3.61	Serotype a	4.01 ± 2.37	Serotype a	2.07 ± 1.45	Serotype a	6.17 ± 3.30
	p = 0.942		p = 0.015		p = 0.419		p = 0.02
Serotype b	24.81 ± 16.44	Serotype b	1.37 ± 2.20	Serotype b	5.82 ± 2.76	Serotype b	3.28 ± 1.78
	p = 0.003		p = 0.788		p = 0.001		p = 0.075
Serotype c	4.21 ± 4.58	Serotype c	1.45 ± 1.37	Serotype c	2.23 ± 1.42	Serotype c	2.18 ± 1.47
	p = 0.922		p = 0.746		p = 0.323		p = 0.454
IL-17	Fold change	TNF-α	Fold change	TLR2	Fold change	TLR4	Fold change
Serotype a	1.16 ± 1.04	Serotype a	2.93 ± 1.56	Serotype a	3.85 ± 1.08	Serotype a	2.31 ± 1.33
	p = 0.802		p = 0.052	10.39 ± 8.83	p = 0.588		p = 0.033
Serotype b	6.00 ± 3.70	Serotype b	6.25 ± 3.29	Serotype c	9.98 ± 5.86	Serotype b	3.46 ± 1.48
	p = 0.001		p < 0.0001		p = 0.005		p = 0.002
Serotype c	1.58 ± 0.97	Serotype c	2.31 ± 1.56	Non induced	1.49 ± 0.99	Serotype c	1.83 ± 1.11
	p = 0.612		p = 0.277	1,00 ± 0.00	p = 0.990		p = 0.116

Table 3 shows the quantitative PCR analysis of IL-1β, IL-4, IL-6, IL-10, IL-17, TNF-α and TLR2 and TLR4 mRNA expression in microglia cell cultures treated with purified LPS (50 ng/mL) of serotypes a, b, or c of Aa. For relative expression, cytokine mRNA expression in non-induced microglia was used as control. Data are presented as fold-change and shown as mean ± SE for 10 independent experiments. Each experiment was performed in duplicate. p values: in comparison to non-induced controls. IL: Interleukin, TLR: Toll-like receptor, TNF: Tumor Necrosis Factor.