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. 2019 Feb 20;24(3):346–361. doi: 10.1177/2472555218823171

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© 2019 Society for Laboratory Automation and Screening

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 2. — (A) Determination of the K_m for the NADPH for TbPTR1 enzyme (K_m 229.1 ± 25.4 µM). The duplicate data from these experiments were fitted using the four-parameter logistic equation using GraphPad Prism (version 5.02, GraphPad Software, Inc.), and standard deviations are within ±10% of the values of the rates. (B) DMSO tolerance. (C) Compound concentration-response curve for TbPTR1 target-based assay against methotrexate (IC₅₀ 2.62 ± 0.12 nM; Hill slope 0.72 ± 0.04), and standard deviations are within ±10% of the values. (D) Summary of compounds evaluated (see Table 1 for details) were shown to inhibit T. brucei growth at 50 µM. See the Materials and Methods section for full experimental details.