Fig. 2.

Effects of CAR10 on invasion and migration of LUAD cells in vitro. a Efficiency of the CAR10 knockdown in A549 and PC9 cells using the independent lncRNA Smart Silencer of CAR10. Data are presented as the mean ± SEM of three independent experiments, two-tailed Student’s t test. b Overexpression of CAR10 (pCDH-CAR10) in A549 and PC9 cells using a lentiviral vector to stably increase CAR10 expression in infected LUAD cells. Mean ± SEM; n = 3 independent experiments, two-tailed Student’s t test. c, d The invasive potential of silenced CAR10 or pCDH-CAR10 A549 and PC9 cells together with their controls was analyzed by a transwell assay. Scale bar: 100 μm. Number of invasive cells was shown as mean ± SEM; n = 3 independent experiments, two-tailed Student’s t test. e–h The migratory potential of CAR10 knockdown or pCDH-CAR10 A549 and PC9 cells together with their controls was analyzed in a wound-healing assay. Scale bar: 200 μM. Distance of the gap was shown as mean ± SEM; n = 3 independent experiments, two-tailed Student’s t test. *P < 0.05, **P < 0.01, and ***P < 0.001. NS no statistical significance