Fig. 3.

CAR10 silencing inhibited tumor initiation/growth and metastasis in vivo. a, b The knockout of CAR10 via a CRISPR/Cas9 system inhibited the growth of A549 cells and subcutaneously transplanted the xenograft into BALB/c nude mice. Data are shown as mean ± SEM; n = 8–9 mice per group, two-tailed Student’s t test. a Tumor formation was monitored at the indicated time points. b Overall tumor growth of the xenografts, and tumor weights were recorded. c CRISPR-CTR or CRISPR-CAR10 A549 cells were intravenously injected into nude mice. Luciferase signal intensities of each group were examined at 8 weeks. d The number of metastatic nodules in the lungs is shown as mean ± SEM (n = 6), using two-tailed Student’s t test. e Representative lungs and hematoxylin and eosin-stained images presented to show metastases in the lungs (scale bar, 200 μm and 50 μm). f Representative lymph nodes and hematoxylin and eosin-stained images of lymph nodes isolated from metastatic tumor mouse models. *P<0.05, **P<0.01, and ***P <0.001. NS no statistical significance