Fig. 6.

CAR10 promoted EMT through miR-30/203-SNAI1/2 axis. a, b The mRNA (a) or protein levels (b) of SNAI1 and SNAI2 increased when CAR10 was inhibited by overexpression of miR-30 or/and miR-203 in A549 cells. Data are shown as mean ± SEM; n = 3 independent experiments, two-tailed Student’s t test. c, d Overexpression of CAR10 enhanced the invasive potential of A549 and PC9 cells; this change was reversed by co-overexpression of miR-30 or/and miR-203. Data are shown as mean ± SEM; n = 3 independent experiments, two-tailed Student’s t test. e Protein levels of SNAI1 and SNAI2 in xenograft tumor tissues of the CRISPR-CTR or CRISPR-CAR10 group detected by western blot assay. f, g The expression of CAR10, SNAI1, SNAI2, and miR-30 and miR-203 was monitored by qRT-PCR in CRISPR-CTR or CRISPR-CAR10 xenograft tumor tissue samples. Data are shown as mean ± SEM; n = 3 independent experiments for three CRISPR-CTR tumor tissue samples and three CRISPR-CAR10 tumor tissue samples, two-tailed Student’s t test. *P < 0.05, **P < 0.01, and ***P < 0.001. NS no statistical significance