Fig. 5.

Neddylation regulates IL-17A-induced synovial cell proliferation and inflammatory responses via NF-κB pathway. a Immunofluorescence staining of phosphorylated p65 in synovium of mice with CIA. b, c Quantification analysis of TNF-α and IL-6 mRNA expression in synovial cells induced by 100 ng/ml IL-17A with or without 10 μM BAY11 pretreatment. d Synovial cell proliferation rate detected by CCK8 assay. e NF-κB transcription activity induced by different doses of NEDD8 in cell line HEK293T. f Western blot analysis of p65 phosphorylation in synovial cells induced by 100 ng/ml IL-17A with or without nedd8 transfection. g Western blot analysis of p65 phosphorylation in synovial cells induced by 100 ng/ml IL-17A with or without 100 nM MLN4924 pretreatment. ****P < 0.0001. P values were analyzed by two-way ANOVA in (b, c) and one-way ANOVA in (d, e). Scale bar represents 50 μm