Fig. 6.

CX-5461 inhibits neuroblastoma xenograft growth in vivo. a Comparison of tumor growth in IMR-32 xenograft bearing mice receiving no treatment (n = 10) or CX-5461 (n = 10) for 3–6 consecutive days and then every third days for 10 days. Tumor volume index (TVI) means with SEM is displayed (****p ≤ 0.0001). b Comparison of tumor growth (tumor volume index; TVI) in BE(2)-C engrafted mice receiving no treatment (n = 9) or CX-5461 (n = 9) every third day for 12 days. TVI means with SEM is displayed (*p ≤ 0.05; **p ≤ 0.01; ****p ≤ 0.0001). c Sections of dissected tumors were analyzed by hematoxylin and eosin (H&E) staining. IMR-32 xenografts sections deriving from mice treated with CX-5461 demonstrated areas with necrotic tissues, which was less evident in corresponding BE(2)-C xenografts, and d immunohistochemical staining with antibodies to detect the apoptotic marker c-Casp-3, e the DNA damage marker γ-H2A.X, f the proliferation marker Ki-67, and g MycN. Scalebar = 100 μm