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In vitro validation of dual-labeling of MDA-MB468. (A) Differential interference contrast (DIC) images, fluorescence images and Prussian blue staining of dual-labeled MDA-MB468. Scales in DIC are 25 μm. (B) Flow cytometory with dextran staining (SPIO staining) of dual-labeled MDA-MB468. Argon ion laser (488 nm) was used for excitation with FL1 emission filter (530/30 nm) for anti-dextran FITC-conjugated antibody and FL3 (650 nm long-pass) for quantum dot 655.
