| 1 cycle: | 2 min | 98°C (initial denaturation) |
| 25 cycles: | 10 sec | 98°C (denaturation) |
| 20 sec | 56.6°C (annealing) | |
| 15 sec | 72°C (extension) | |
| 1 cycle: | 5 min | 72°C (final extension) |
| Final step: | Indefinite | 4°C (hold). |
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