Figure 3.

Ethanol exposure of hiPSC-CMs increases ROS production. A, Detection of intracellular ROS production. Human-induced pluripotent stem cell-derived cardiomyocytes were stained with DCFDA to detect ROS production upon ethanol exposure for 5 days. Reactive oxygen species-positive cells exhibited bright green cytosolic fluorescence and nuclei were stained with Hoechst. B, Detection of mitochondrial ROS production through ArrayScan. Human-induced pluripotent stem cell-derived cardiomyocytes were stained with MitoSOX Red to detect mitochondrial ROS production upon ethanol exposure for 5 days. Data presented as average fluorescence intensity per well and percentage of MitoSOX Red-positive cells, shown as mean±SD (n = 5). NS, no significant difference compared with control (0 mM). **p-value<.01; ****p-value < .0001. Representative images are presented in Figure S2.