Fig. 3.

The intracellular pathways associated with RANK-RANKL signaling and IL-8 production were evaluated with and without anti-RANKL Ab. Peripheral monocytes cultured with M-CSF and RANKL for 10 days had their media changed and were then incubated with M-CSF only, M-CSF and RANKL, and M-CSF and RANKL with anti-RANKL Ab at 37°C for 1 h. The MFIs of phosphorylated (p) ERK, NF-κB and Src were measured using a flow cytometer. Representative images from five RA patients are shown. Red, blue, green and gray lines represent M-CSF and RANKL with anti-RANKL Ab, M-CSF only, M-CSF and RANKL, and unstained, respectively. Additionally, the median MFIs for pERK, pNF-κB and pSrc were also evaluated (n = 5). Error bars indicate SD. Statistical significance was evaluated using paired Student’s t-test.