Fig. 4. The effects of compound 6k on the nuclear translocation of the NF-κB protein induced by cytokine TNF-α. After culturing overnight, A549 cells were pretreated with 6k (10 μM) for 24 hours, followed by TNF-α (10 ng mL^–1) for 30 min, and then processed for NF-κB nuclear translocation detection by immunofluorescence staining.