Table 2. Cytotoxicity of compounds 5a–5h and 6a–6l against different cancer cell lines.
| Compd. | IC50
a
(μM) |
RF c | |||||
| T24 | HepG2 | A549 | NCI-H460 | NCI-H460/DOX | HL-7702 | ||
| 5a | 27.39 ± 1.15 | 29.76 ± 0.57 | 26.71 ± 1.06 | 26.84 ± 0.36 | n.d. b | >50 | — |
| 5b | 33.82 ± 0.63 | 37.55 ± 0.88 | 33.47 ± 1.21 | 34.63 ± 0.99 | n.d. | >50 | — |
| 5c | 20.27 ± 0.54 | 25.12 ± 1.25 | 18.51 ± 1.34 | 20.78 ± 0.85 | >50 | >50 | — |
| 5d | 39.28 ± 1.26 | 43.75 ± 1.48 | 38.23 ± 1.26 | 40.91 ± 1.37 | n.d. | >50 | — |
| 5e | >50 | >50 | >50 | >50 | n.d. | >50 | — |
| 5f | 22.47 ± 1.23 | 26.93 ± 0.91 | 21.85 ± 0.68 | 23.50 ± 0.59 | n.d. | >50 | — |
| 5g | 17.33 ± 1.09 | 21.48 ± 0.78 | 16.74 ± 0.93 | 19.06 ± 0.76 | 27.11 ± 1.05 | >50 | 1.42 |
| 5h | 31.95 ± 0.43 | 36.84 ± 0.96 | 30.26 ± 0.87 | 34.49 ± 1.14 | n.d. | >50 | — |
| 6a | 23.62 ± 1.35 | 28.42 ± 1.03 | 20.36 ± 0.62 | 24.96 ± 1.46 | n.d. | >50 | — |
| 6b | 34.49 ± 1.12 | 37.29 ± 1.07 | 33.15 ± 0.98 | 34.88 ± 0.75 | n.d. | >50 | — |
| 6c | 9.10 ± 0.74 | 10.32 ± 0.45 | 8.98 ± 0.56 | 9.65 ± 0.29 | 14.43 ± 0.58 | >50 | 1.49 |
| 6d | >50 | >50 | >50 | >50 | n.d. | >50 | — |
| 6e | 37.69 ± 0.71 | 40.58 ± 0.87 | 35.68 ± 1.25 | 39.73 ± 0.79 | n.d. | >50 | — |
| 6f | 41.14 ± 2.58 | 45.80 ± 1.80 | 39.87 ± 1.55 | 43.57 ± 1.64 | n.d. | >50 | — |
| 6g | 14.95 ± 1.15 | 18.71 ± 0.90 | 14.78 ± 0.32 | 15.92 ± 0.57 | 17.41 ± 0.34 | >50 | 1.09 |
| 6h | >50 | >50 | >50 | >50 | n.d. | >50 | — |
| 6i | 9.61 ± 0.63 | 12.66 ± 0.86 | 9.24 ± 1.14 | 11.74 ± 0.37 | 13.99 ± 0.95 | >50 | 1.19 |
| 6j | 10.67 ± 0.72 | 14.87 ± 0.37 | 10.20 ± 0.49 | 12.05 ± 0.84 | 16.92 ± 1.01 | >50 | 1.40 |
| 6k | 3.96 ± 0.21 | 5.24 ± 1.34 | 2.67 ± 0.06 | 4.46 ± 0.15 | 4.84 ± 0.42 | >50 | 1.08 |
| 6l | 7.51 ± 0.54 | 8.36 ± 1.11 | 5.16 ± 0.51 | 7.85 ± 0.83 | 10.27 ± 0.35 | >50 | 1.31 |
| AA | 43.97 ± 1.36 | 35.37 ± 1.47 | 41.02 ± 0.78 | 44.82 ± 1.25 | n.d. | >50 | — |
| DOX | 0.96 ± 0.09 | 1.37 ± 0.24 | 1.07 ± 0.18 | 0.8 ± 0.06 | 30.99 ± 1.15 | 10.44 ± 0.5 | 38.74 |
aIC50 values are presented as the mean ± SD (standard error of the mean) from three separate experiments.
bn.d. = not detected.
cRF (resistant factor) is defined as IC50 in NCI-H460/DOX/IC50 in NCI-H460.