Fig. 2.

Comparison of the mitotic spindle acquired with (a) confocal and (b) 2D STED. Images are displayed with the cubehelix colormap. The insets in (a) and (b) show the zoomed area marked with the dashed rectangle. The plot in (c) shows the intensity cross-section at the position shown by the yellow line in the zoomed images in (a) and (b). The blue plot shows the cross-section through the confocal image, red is the cross-section through 2D STED image and the green plots represents the Lorentzian fit to the 2D STED cross-section. The slices shown are at a depth of 5µm from the bottom of the mitotic cell. The scale bar is 1µm.