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. 2019 Mar 15;10(4):1856–1869. doi: 10.1364/BOE.10.001856

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Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article's title, journal citation, and DOI.

Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article's title, journal citation, and DOI.

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Fig. 7 — (Top) Typical ex vivo imaging of lung tissue from EVLP model in alveolar space before and after in situ instillation of pre-labelled bacteria. Greyscale images show how imaging appears in the widefield imaging system without spectral ratio analysis enabled. Coloured images display spectral ratio and intensity with rescaling of spectral ratio to 0.3 - 0.4 to enhance SmartProbe labelled P. aeruginosa visibility. (Bottom) Typical histogram of spectral ratio across 300 frame videos of lung before and after delivery of labelled bacteria. A tabulated series of images can be seen in Fig. 6.