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. 2019 Feb 28;9(6):1683–1697. doi: 10.7150/thno.30487

Figure 2.

Figure 2

Differentiation of neural cells with spinal cord identity from hiPSC-NMP. A. Schematic representation of the differentiation conditions used to generate neuroprogenitors and neurons with spinal cord identity from hiPSC-NMP. Scale bar: 100 μm. B. Immunofluorescence staining for the neuroprogenitor markers, PAX6, SOX2, and NESTIN, in hiPSC-NMP that had been treated with dSMADi/RA for 6 days. Scale bar: 100 μm. C. Motor neuron markers (ISL1, HB9, and TUBB3) and a cervical spinal cord marker (HOXC6) were detected by immunostaining. Scale bar: 100 μm.