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Generation of paraxial mesoderm from hiPSC by extended treatment with bFGF, TGFβ1, and Chir99021. A. hiPSC were treated with bFGF, TGFβ1, and Chir99021 for 4-5 days and observed under phase-contrast microscopy, which showed that cells proliferated quickly and became confluent by day 4. Scale bar: 100 μm. B. Immunostaining was used to detect paraxial mesoderm markers (T and TBX6) and SOX2. Scale bar: 100 μm.
