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. 2019 Feb 28;9(6):1666–1682. doi: 10.7150/thno.27891

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Biochemical and morphology analysis of B16F10 exosomes. (A) Detection of exosomal markers CD81 and CD9 using flow cytometry on exosomes isolated from B16F10 cells. Exosomes were coupled to aldehyde/sulphate latex beads prior to detection. Exo-beads complex were subsequently stained using a 2-step labelling (anti-CD81 or anti-CD9 1° ab/Cy5-conjugated 2° ab). Degree of expression of the markers are expressed as the fold difference in median fluorescence intensity (MFI) values from that of the control (exo-beads complex stained with Cy5-conjugated 2° ab). Values are expressed as mean ± SD, where n=3. (B) Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) images of exosomes isolated from B16F10 cells.