Table 1.

Physicochemical characterisation of exosomes

Exosome	Size1,2 (nm)	Yield1,2,3 (p/ml)	[Protein]2,4 (µg/ml)	Particle to protein (P:P) ratio2,5 (p/µg)
B16F10	132.3 ± 5.6	2.04x1013 ± 3.9x1012	451.15 ± 71.5	4.52x1010 ± 1.26 x1010

¹Measured using NanoSight LM10.

²Values are expressed as mean ± SD, where n=3.

³Yield was obtained by cell-conditioned medium pooled from 6 rounds of harvesting from CELLine AD1000 flasks (72 ml).

⁴Measured using BCA assay.

⁵Value obtained by using formula: P:P ratio = Yield / [Protein].