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. 2019 Feb 28;9(6):1698–1713. doi: 10.7150/thno.30640

Figure 3.

Figure 3

BBR inhibited PA-induced mitochondrial fragmentation in podocytes. (A) Mitochondrial morphology was observed by TEM and assessed by aspect ratio and form factor using Image J from three independent experiments (>100 mitochondria). Scale bars, 500 nm. (B) Aspect ratio, form factor, circularity, and roundness were quantified for each condition (>100 mitochondria). (C) Feret's diameter that reflects the longitudinal mitochondrial length was measured by Image J analysis (>100 mitochondria). (D) Quantification of the mitochondrial morphology shown in (E) by Image J analysis from three independent experiments (>500 mitochondria). (E) Mitochondria were stained by MitoTracker Red and imaged by laser scanning confocal microscopy. Scale bars, 10 μm. Error bars represent mean ± SEM, except morphological parameters of mitochondria shown as the median (interquartile range). *P < 0.05 vs. Control; #P < 0.05 vs. PA. BBR, Berberine; PA, palmitate; TEM, transmission electron microscope.