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. 2019 Apr 23;7:e6807. doi: 10.7717/peerj.6807

Figure 4. Immunohistochemistry (cytoplasmic, positive) of CYP2J2 and CYP4A (magnification, ×200).

Figure 4

(A) Positive staining for CYP2J2 (A–H) was observed in the renal cortex and outer medulla of all groups. Under the normal diet, the positive reaction of D group was enhanced compared to B group. Under the high-salt diet, the positive reaction and staining intensity of G and H groups were increased compared to E and F groups. In tissue sections with arrow marks, the arrow a indicated the cortical staining area and the arrow b indicated the outer medulla stained area. Renal sections of all groups were observed to be positively stained for CYP4A (I–P) in interlobar, arcuate, and interlobular arteries. However, no positive enhancement was observed in the sections. (B) Under the normal diet, renal CYP2J2 expression (Q) was significantly elevated in CYP2C11-null females compared with WT females (P < 0.01, n = 5). We observed the same between-strain difference under the high-salt diet, except in both sexes (P < 0.01, n = 5). No significant difference was found for CYP4A expression (R). Density (mean) = integrated optical density (IOD)/Area. All data were expressed as means ± standard deviation. **P < 0.01.