Table 1. Detailed information of 15 candidate reference genes evaluated in this study.
| Genes | Full name | Accession No. | Position | Length (bp) | Amplification efficiency |
|---|---|---|---|---|---|
| Rps18 | 18S Ribosomal RNA | NM_213557 | exon2/exon3 | 140 | 1.02 |
| Actb | ß-actin | NM_031144 | exon3/exon4 | 165 | 1.05 |
| B2m | ß-2-microglobuIin | NM_012512 | exon2/exon3, 4 | 114 | 1.06 |
| Gapdh | Glyceraldehyde-3-phosphate dehydrogenase | NM_017008 | exon6/exon7 | 92 | 0.99 |
| Ppia | Peptidylprolyl isomerase A | NM_017101 | exon1/exon3 | 133 | 1.04 |
| Hprt1 | Hypoxanthine phosphoribosyl transferase 1 | NM_012583 | exon8/exon9 | 105 | 1.02 |
| Pgk1 | Phosphoglycerate kinase 1 | NM_053291 | exon8/exon9 | 104 | 1.03 |
| Ywhaz | Tyrosine 3-monooxygenase | NM_013011 | exon2/exon3 | 127 | 1.00 |
| Tbp | TATA binding protein | NM_001004198 | exon4/exon5 | 123 | 1.01 |
| Ubc | Ubiquitin C | NM_017314 | exon3 | 82 | 0.99 |
| Rps16 | 16S Ribosomal RNA | NM_001169146.1 | exon1/exon3 | 147 | 1.05 |
| Rpl13a | Ribosomal protein L13A | NM_173340 | exon5/exon7 | 131 | 1.01 |
| Rplp1 | 60S acidic ribosomal protein large PI | NM_001007604 | exon2/exon3 | 87 | 0.98 |
| Sdha | Succinate dehydrogenase complex, subunit A, flavoprotein (Fp) | NM_130428 | exon9/exon10 | 105 | 0.99 |
| Hmbs | Hydroxymethylbilane synthase | NM_013168 | exon13/exon14 | 76 | 1.04 |
Note:
Accession No., NCBI accession number. PCR amplification efficiency = 10−1/slope − 1.