Table 4. Descriptive statistics for 10 candidate reference genes obtained by BestKeeper analysis.
| Genes | N | GM (Ct) | AM (Ct) | Min (Ct) | Max (Ct) | SD (±Ct) | CV (% Ct) | r with BKI | p-value | Ranking order |
|---|---|---|---|---|---|---|---|---|---|---|
| Rpl13a | 18 | 14.395 | 14.400 | 13.738 | 15.057 | 0.333 | 2.310 | 0.921 | 0.001 | 1 |
| Actb | 18 | 15.094 | 15.104 | 14.029 | 16.152 | 0.447 | 2.961 | 0.892 | 0.001 | 2 |
| Ywhaz | 18 | 17.283 | 17.288 | 16.617 | 18.134 | 0.349 | 2.017 | 0.891 | 0.001 | 3 |
| Sdha | 18 | 21.886 | 21.890 | 21.087 | 23.017 | 0.360 | 1.645 | 0.876 | 0.001 | 4 |
| Rps18 | 18 | 14.883 | 14.886 | 14.236 | 15.355 | 0.214 | 1.438 | 0.786 | 0.001 | 5 |
| Hmbs | 18 | 21.373 | 21.376 | 20.691 | 21.999 | 0.327 | 1.530 | 0.726 | 0.001 | 6 |
| Rplp1 | 18 | 14.068 | 14.072 | 13.609 | 14.948 | 0.278 | 1.973 | 0.707 | 0.001 | 7 |
| Gapdh | 18 | 16.052 | 16.057 | 15.320 | 16.950 | 0.340 | 2.118 | 0.696 | 0.001 | 8 |
| Ubc | 18 | 16.568 | 16.569 | 16.075 | 16.898 | 0.192 | 1.158 | 0.550 | 0.018 | 9 |
| Hprt1 | 18 | 21.357 | 21.360 | 20.740 | 22.278 | 0.320 | 1.500 | 0.510 | 0.031 | 10 |
Note:
N, sample numbers; Ct, cycle threshold value; GM, geometric; AM, arithmetic; SD, standard deviation; CV, coefficient of variation; r, Pearson correlation coefficiency; BKI, BestKeeper Index; p-value, p-values associated with Pearson correlation.