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The construction of pLKO.1-MSI-1-S2 expression Vector. (A)Map of pLKO.1-MSI-1-S2 recombinant plasmid and its restriction site. (B) Small hairpin S2 schematic diagram. The U6 promoter guides transcription of small hairpin S2; includes 23 sense bases and 23 antisense bases of S2.