Figure 1.

The AnchorIRIS^TM assay and performance assessment. (A) Workflow of the ultra-sensitive AnchorIRIS^TM library preparation method. (B-C) A bake-off experiment comparing assay performance between the AnchorIRIS^TM assay and the SWIFT^® accel-NGS Methyl-seq^TM assay. The IRIS assay presents superior molecule conversion efficiency (C) with much higher average unique coverage for each input amount tested (B). (D and E) The sensitivity of the AnchorIRIS^TM assay was evaluated by diluting tumor gDNA into WBC gDNA, showing that significantly more informative co-methylated CpG regions above WBC background can be detected at dilutions ≥ 0.033% by Z-test (D). Dilutions higher than 10% (gray box) preserve a linear response of average co-methylation signal to the tumor fractions of input DNA (E).