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. 2019 Apr 8;50(5):1232–1239. doi: 10.1161/STROKEAHA.118.023620

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© 2019 The Authors.

Stroke is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited.

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Figure 2. — Immune cell recruitment and pro–IL (interleukin)-1β expression in immune cells 24 h after stroke. A, Gating strategy and flow cytometry analysis of microglia/macrophages CD45^int-hi/CD11b^hi/CD64^hi, monocytes CD45^hi/CD11b^hi/Ly6C^hi, and neutrophils CD45^hi/CD11b^hi/Ly6G^hi in the ipsilateral hemisphere. B, (i) Percentage of microglia/macrophages, monocytes, and neutrophils vs the total number of live cells in the contralateral and ipsilateral hemisphere. B, (ii) Absolute number of microglia/macrophages, monocytes, and neutrophils in the contralateral and ipsilateral hemisphere (n=4 mice, *P<0.05, **P<0.01, analysed by 2-tailed paired t test). C, Gating strategy analysis and flow cytometry analysis of pro–IL-1β 24 h after stroke. D, (i) Percentage of pro–IL-1β positive cells and (D, ii) geometric mean fluorescence intensity of pro–IL-1β staining in microglia/macrophages, monocytes, and neutrophils (n=6 mice, *P<0.05, **P<0.01, analysed by 2-tailed paired t test). FMO indicates fluorescence minus one; FSC-A, forward scatter–area; FSC-H, forward scatter–height; SSC-A, side scatter–area.