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. 2019 Apr 8;50(5):1232–1239. doi: 10.1161/STROKEAHA.118.023620

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© 2019 The Authors.

Stroke is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited.

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Figure 3. — Influence of NLRP3 (NACHT, LRR and PYD domains-containing protein 3) inhibition on brain damage after stroke. A, (i) Schematic representation of experiment. A, (ii) representative cresyl violet staining and (iii) lesion volume quantification 24 h after stroke in mice treated with MCC950 (50 mg/kg) or saline by intraperitoneal injection 30 min after the stroke onset (n=6/group). B, (i) Representative immunostaining of IL (interleukin)-1β (green), neutrophils (Ly6G, red) and 4’,6-diamidine-2’-phenylindole dihydrochloride (DAPI; blue) in the infarct 24 h after stroke (scale bar in the large image is 100 μm and in the inset is 20 μm). B, (ii) Numbers of neutrophils and IL-1β positive cells per mm², and % IL-1β positive neutrophils in the infarct 24 h after stroke plus and minus intraperitoneal MCC950 (n=3–4/group). C, (i) Schematic representation of the experiment. C, (ii) Representative cresyl violet staining and (iii) lesion volume quantification 24 h after stroke in mice treated with MCC950 (10 μg) or saline by intracerebroventricular injection 30 min after stroke onset (n=7–8/group). D, (i) Representative immunostaining of IL-1β (green), neutrophils (Ly6G, red) and DAPI (blue) in the infarct 24 h after stroke (Scale bar in the large image is 100 μm, and in the inset is 20 μm). D, (ii) Numbers of neutrophils and IL-1β positive cells per mm², and % of IL-1β positive neutrophils in the infarct 24 h after stroke plus and minus intracerebroventricular MCC950 (n=7/group). Stroke was induced by middle cerebral artery occlusion (MCAo) using FeCl₃.