Figure 1.

The effect of GFP (green fluorescent protein) conjugation to the N and C terminus of troponin subunits on myofilament function. Myofilament function was assessed using in vitro actin-activated actomyosin S1 ATPase assays. Control (unconjugated) troponin complexes (gray lines) were compared pairwise to troponin complex reconstituted with subunits conjugated to the N or the C terminus of GFP as illustrated (red lines). n=3–5 error bars are±SEM. TnC indicates Troponin C; TnI, Troponin I; and TnT, Troponin T.