Figure 6.

The effects of levosimendan on Ca²⁺ transient measurements in Guinea pig cardiomyocytes (GPCMs) with RGECO, RGECO-TnT (Troponin T), or RGECO-TnI (Troponin I). Averaged Ca²⁺ transients of 0.5 Hz paced GPCMs transduced with RGECO, RGECO-TnT, or RGECO-TnI was used to compare the effects 10 μmol/L levosimendan (A). Each comparison is made in paired experiments between drug treated (solid lines) and DMSO control treated (dashed lines) for RGECO infected (red), RGECO-TnT infected (purple), and RGECO-TnI infected (blue) cells. Dot plots for all extracted parameters are plotted in (B; n=71–83 cells from n=3 isolations). Lines are median average and error bars are interquartile range, *P<0.05, **P<0.01, and ***P<0.001 using an unpaired Mann-Whitney test comparing untreated to treated cells.