Figure 6.

Tamoxifen treatment of Cre^+/− β3^flox/flox mice decreases β3 integrin expression. Anterior segments of Cre^+/− β3^flox/flox or Cre^−/− β3^flox/flox mice treated with or without tamoxifen were lysed and analyzed by Western blotting for levels of β3 integrin or the housekeeping gene GAPDH. (A) Densitometry of β3 integrin bands were normalized to GAPDH levels. (B) Ratio expressed as a percentage of tamoxifen versus control from Cre^+/− β3^flox/flox or Cre^−/− β3^flox/flox mice in (A). (C) Real-time PCR of β3 integrin using gDNA isolated from Cre^+/− β3^flox/flox or Cre^−/− β3^flox/flox mice treated with or without tamoxifen. Levels of β3 integrin in Cre^+/− β3^flox/flox mice treated with tamoxifen were significantly different from those in control Cre^+/− β3^flox/flox mice (*P < 0.05). Tam, tamoxifen, Cont, control. Error bars are mean ± SEM.