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. Author manuscript; available in PMC: 2019 Apr 26.
Published in final edited form as: Sci Signal. 2018 Jun 19;11(535):eaao2060. doi: 10.1126/scisignal.aao2060

Fig. 4. Piezo2 MA current modulation by ANXA6 coexpression.

Fig. 4

ND-C cells were cotransfected with hPIEZO2-IRES-eGFP and/or hANXA6-pIRES2-tdTomato or their respective EVs IRES-eGFP and pIRES2-tdTomato according to the indicated combinations, and mechanotransduction was assessed by the mechano-clamp technique. (A) Membrane capacitance of cells transfected as indicated. (B) Minimal stimulation intensity required to elicit a >40-pA MA current in cells transfected as indicated. (C) Maximum recoded MA current density in cells transfected as indicated. (D) Current density plotted against the displacement of the mechanical probe in cells transfected as indicated. (A to D) *P < 0.05, **P < 0.01, ***P < 0.001 compared to PIEZO2 + EV by one-way ANOVA followed by Fisher’s LSD test (A to C) or two-way ANOVA followed by Fisher’s LSD multiple comparisons test (D). n = 4 EV + EV, 6 EV + ANXA6, 10 PIEZO2 + EV, and 5 PIEZO2 + ANXA6 cells.