Extended Data Figure 7. PGCLC induction of independent Blimp1-null cell lines.

a. Scheme showing the strategy used to generate Blimp1 KO cell lines. A pair of gRNAs flanking Blimp1 exon5 were co-expressed to ensure complete deletion of Blimp1 exon5. Red arrows represent genotyping primer pairs used to screen clones.

b, c. Blimp1-null clones used in Figure 3 (b) or Extended Figure 8d (c) were genotyped using primers indicated in a. n= 2 biologically independent replicates for both, all clones have been sequenced.

d. Cells of the indicated genotypes (c) were assessed by flow cytometry for surface expression of SSEA1 and CD61 at day 6 of aggregation in the presence of PGC induction cytokines. n=2

e-f. Cells of the indicated genotypes (c) were assessed by flow cytometry for surface expression of SSEA1 and CD61 at day 6 of aggregation in the absence of PGC induction cytokines. n=2