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. Author manuscript; available in PMC: 2019 Apr 26.
Published in final edited form as: Nature. 2018 Oct 3;562(7728):595–599. doi: 10.1038/s41586-018-0581-5

Figure 4. Otx2-/- ESCs contribute to the germline at an enhanced rate in vivo.

Figure 4

a. Scheme for PGCLC differentiation, initiated from day 4 EpiLCs obtained after 1 passage from EpiLCs.

b. Representative morphologies and Oct4ΔPE::GFP expression from aggregates at day 6 of PGCLC differentiation from EpiLCs day4 (n=3 for 1 clone of each genotype). Bar = 200μm.

c. Scheme for generating chimaeras of GFP labelled Otx2+/+ or Otx2-/- ESCs with wildtype host embryos.

d. Comparison of the percentage contribution of GFP labelled wildtype (n=6) or Otx2-null ESCs (genotypes indicated, n=9 for each) to the PGC population in E7.5 chimaeric embryos. Each dot represents the percentage from one chimaera, centre lines and error bars represents means±SD. P value (two-sided unpaired T-test, 0.95 confidence intervals) is indicated. GFP positive cells were counted within the PGC population marked with BLIMP1 or SOX2 in each embryo.

e. Comparison of PGCs number in wild-type (n= 4) and Otx2-/- (n= 3) E7.5 embryos. PGCs were identified with Blimp1, AP2γ and Fragilis. Values= means ± SD.

f. Model indicating the point of operation of OTX2 during germline-soma segregation.

g. A scheme illustrating the regulatory relationships upstream and downstream of Otx2 during germline segregation.