Skip to main content
. Author manuscript; available in PMC: 2019 Apr 26.
Published in final edited form as: Cancer Res. 2009 Dec 15;69(24):9219–9227. doi: 10.1158/0008-5472.CAN-09-1852

Figure 5. Exon 2-containing TP53INP2 transcripts are required to support Golgi morphology and invasive migration of tumour cells.

Figure 5

(a-c) A2780 cells were transfected with either control (Con) siRNAs or those targetting exon 2-containing TP53INP2 transcripts (E2), in combination with a fluorescently-tagged Golgi marker and seeded onto cell-derived matrix, and their Golgi morphology analysed and quantified as for figure 2 c&d. Bar in a, 50 μm. Bar in b, 20μm. Data represents mean ± SEM. * indicates statistical significance of p=<0.0001 and ** indicates p<0.05 (Mann-Whitney test).

(d) A2780 or BE cells were transfected with either control (Con) siRNAs or those targetting exon 2-containing TP53INP2 transcripts (E2) and their invasion into plugs of Matrigel supplemented with fibronectin was determined using an inverted invasion assay. Invasion assays were quantitated by measuring the proportion of cells within the plug that penetrate the Matrigel to depths of >30 μm (for A2780 cells) and >45 μm (for BE cells). Data represent mean ± SEM, n>4. ** indicates significant difference of p<0.05 (Mann-Whitney test for non-parametric data).