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. Author manuscript; available in PMC: 2019 Apr 26.
Published in final edited form as: FASEB J. 2007 Jul 11;21(14):4095–4100. doi: 10.1096/fj.06-7927com

Figure 2.

Figure 2

Co-immunoprecipitation of MC2R-Nup50 complex in H295R cells. (a) Both the C16 and H-70 antibodies raised against the MC2R precipitate a similar sized 35S-methionine-labelled band in mouse Y1 cells. (b) Using both antibodies it was possible to co-immunoprecipitate Nup50 in H295R cells. (c) Nup 62 does not interact with MC2R. Immunoprecipitation of buffer alone (lane 1) or H295R lysate (lane 2) with the H70 MC2R antibody failed to precipitate Nup 62. Nup 62 is readily detectable in non-immunoprecipitated cell lysates (lane 3).