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Effect of inhibition of signal transduction. Nuclear fractions of H295R cells stimulated with or without ACTH (10−6M) for 90 min with prior exposure to either the protein kinase A inhibitor (H89) or the MAP kinase inhibitor (UO126) were used to coimmunoprecipitate the MC2R-Nup50 complex. Neither inhibitor appears to significantly reduce appearance of the complex.
