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. Author manuscript; available in PMC: 2019 Apr 26.
Published in final edited form as: Circulation. 2016 May 9;133(24):2391–2403. doi: 10.1161/CIRCULATIONAHA.116.021993

Figure 4. NADPH oxidase subunit expression, Nox2 maturation and binding to p22phox in HPMEC.

Figure 4

A) Western blots. Optical densities (OD) of protein bands of p22phox, Nox1, Nox2, Nox4, p47phox, p67phox, p40phox and Rac1/2 were quantified and normalized to the levels of α-tubulin (loading control) detected in the same samples. (B) Flow cytometry for the binding of Nox2 antibody (7D5). *p<0.05 for indicated values versus to vector values. †p<0.05 for indicated values versus WT values. (C) Left panel: p22phox was immunoprecipited (IP) followed by immunoblotting (IB) of Nox1, Nox2 and Nox4. Right panels: Optical densities (OD) of protein bands were quantified and normalized to the levels of total p22phox detected in the same samples. *p<0.05 for indicated values versus vehicle values under the same gene transfection. †P<0.05 for indicated values versus WT TNFα values. n=4 independent experiments.