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. Author manuscript; available in PMC: 2019 Apr 26.
Published in final edited form as: Dev Cell. 2016 Feb 8;36(3):249–261. doi: 10.1016/j.devcel.2016.01.007

Figure 4. E-Cadherin, F-actin and pMyosin localization and myosin dynamics before and during gastrulation movements.

Figure 4

(A and B) Transverse cryosections of stage 3 (A) and stage X (B) embryos stained with Phalloidin (A and B), pMyosin (A’ and B’) and E-Cadherin (A” and B”) antibodies. (A”’ and B”’) show the merged pictures.

(C-J) Confocal ortho-slices of stage 3 (C-F) and stage X (G-J) whole mount embryos stained with Phalloidin, pMyosin and E-Cadherin antibodies. Arrows point at F-actin and pMyosin accumulation, asterisks show free-contact cell interfaces, arrowheads point at basal E-Cadherin junctions.

(K) Left panel: time series of a GFP-Myosin electroporated cell showing localization at the cortex and at the cytokinetic furrow in a stage 3 embryo. Right panel: Example of images used for GFP-Myosin (heatmap color code) FRAP experiments, showing the region used for FRAP (arrowhead) of epithelial cells in stage X and 3 embryos.

(L) Left panel: FRAP curves of cortical GFP-Myosin in stage X (red) and stage3 (blue) embryos, errors bars indicate SEM, n= 30 and 61 for stage X and stage 3, respectively. Right panel: Common plot of mobile fraction for all FRAP experiments done at stage X and 3; associated average numbers of the mobile fraction of each fitted curve and statistical significance between the two stages obtained by a Mann Whitney t test, p-value < 10-5(****).

Scale bar is 10μm. See also Figure S2, S3 and S4.