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. Author manuscript; available in PMC: 2019 Apr 26.
Published in final edited form as: FEMS Microbiol Lett. 2005 Sep 28;253(1):89–94. doi: 10.1016/j.femsle.2005.09.021

Fig. 1.

Fig. 1

Schematic representation of the plasmids used in this study. The recombinant plasmid pMM20-1 corresponds to the pBluescript KS vector backbone (double line) ligated to the tetM determinant (thick single line) and to a 6.9 kb DNA fragment of M. agalactiae (thin single line) that carries the dnaA gene. Plasmids pMMΔ oriC, pMM21-4 and pMM21-7 were derived from pMM20-1 by total or partial deletion of the insert. Numbers mentioned below the dotted arrows indicate the approximate size of the fragments and bla designates the ampicillin resistance gene. Restriction enzymes: C, ClaI; E, EcoRI; EV, EcoRV; H, HindIII; N: NotI; P, PstI; S, SalI; Sm, SmaI; X, XbaI.