Figure 5.

Light micrographs of basil glands isolated from line EMX-1. A, Mixture of glandular trichomes prior to final purification step, showing the difference in morphology between the peltate (1) and capitate gland (2) types in basil. Some non-glandular hairs are also visible (3), as are oil sac ghosts (4) and a few broken glands (5). Scale = 20 μm. B, Purity of the peltate glands after final purification, scale = 80 μm. C and D, Isolated peltate glands, not stained with toluidine blue, with focus set at the interface between the gland disc cells and the overlying oil sac (C) and through the middle of the disc of secretory cells (D), scale = 20 μm. E and F, Isolated peltate glands stained with toluidine blue, showing the prominent nucleoli (E) and a comparison of the cross-sectional and transverse views of the disc of secretory cells (F), scale = 20 μm. G, Purity of the capitate glands after final purification, scale = 80 μm.