Table 2.
Top 15 pathways altered by 5-day bed rest
| Young |
Old |
||||
|---|---|---|---|---|---|
| Pathway | −log(P value) | z Score | Pathway | −log(P value) | z Score |
| Mitochondrial Dysfunction (72) | 36.6 | NaN | Hepatic Fibrosis/Hepatic Stellate Cell Activation (49) | 14.8 | NaN |
| Oxidative Phosphorylation (56) | 34.3 | NaN | Epithelial Adherens Junction Signaling (40) | 12.5 | NaN |
| Actin Cytoskeleton Signaling (42) | 7.85 | −0.164 | Actin Cytoskeleton Signaling (49) | 11 | −3.479 |
| Estrogen Receptor Signaling (28) | 7.01 | NaN | Calcium Signaling (41) | 10.1 | 1.291 |
| Epithelial Adherens Junction Signaling (29) | 6.29 | NaN | ILK Signaling (40) | 8.32 | −1.897 |
| Glucocorticoid Receptor Signaling (44) | 5.74 | NaN | Axonal Guidance Signaling (68) | 7.6 | NaN |
| Calcium Signaling (31) | 5.34 | 1.213 | Tight Junction Signaling (31) | 5.67 | NaN |
| RhoA Signaling (24) | 5.11 | −2.132 | Cellular Effects of Sildenafil (26) | 5.41 | NaN |
| ILK Signaling (32) | 4.94 | −1.061 | Germ Cell-Sertoli Cell Junction Signaling (31) | 5.34 | NaN |
| TR/RXR Activation (20) | 4.72 | NaN | Signaling by Rho Family GTPases (39) | 5.1 | −1.372 |
| Estrogen-Dependent Breast Cancer Signaling (17) | 4.56 | 1.000 | Sertoli Cell-Sertoli Cell Junction Signaling (31) | 5.07 | NaN |
| PAK Signaling (20) | 4.52 | −1.213 | Mitochondrial Dysfunction (30) | 5 | NaN |
| PPARα/RXRα Activation (29) | 4.43 | −2.502 | RhoGDI Signaling (30) | 4.89 | 1.732 |
| Integrin Signaling (33) | 4.34 | −0.898 | RhoA Signaling (24) | 4.85 | −2.558 |
| NRF2-Mediated Oxidative Stress Response (30) | 4.26 | −1.155 | Oxidative Phosphorylation (22) | 4.8 | NaN |
n = 9 Young, 18 Old. Number of genes regulated within each pathway in parentheses. Statistical design included age + age:nested + age:time. Fold changes were determined with maximum-likelihood estimates of the log2 fold change. Boldface indicates a pathway that was altered uniquely within that age group. NaN, z score could not be calculated. An adjusted P value of P < 0.05 was required for a gene to be considered significantly altered.