Table II.
Endogenous levels of BRs in cultured zinnia cells and medium during differentiation
| BRs | BR Level
|
||||||
|---|---|---|---|---|---|---|---|
| 0 h
|
30
h
|
54 h
|
78 h
|
||||
| Cells | Cells | Medium | Cells | Medium | Cells | Medium | |
| ng g−1 fresh wt | ng L−1 | ng g−1 fresh wt | ng L−1 | ng g−1fresh wt | ng L−1 | ||
| BL | NDa | ND | ND | ND | ND | ND | ND |
| CS | 0.2 | 0.2 | ND | 0.5 | 25.2 | 0.6 | 31.7 |
| TY | ND | ND | 0.4 | 3.3 | 30.0 | 3.8 | 36.0 |
| TE | ND | ND | ND | ND | ND | ND | ND |
| 6-DeoxoCS | 1.8 | 9.4 | 10.0 | 44.5 | 58.6 | 21.0 | 30.5 |
| 6-DeoxoTY | 0.4 | 8.5 | 1.5 | 320.5 | 31.0 | 289.7 | 25.0 |
| 6-DeoxoTE | 0.3 | 0.3 | 2.0 | 1.2 | 1.7 | 1.0 | 1.4 |
The levels of BRs were determined by GC-MS using deuterated internal standards.
a
ND, Not detected.