Fig. 1.

DjChE shows kinetic characteristics intermediate to mammalian AChE and BChE. a Activity of DjChE was determined using an Ellman assay over a range of ATCh (solid black line) and BTCh (dashed gray line) concentrations. Activity is reported as the percent of the maximum activity in that experiment using ATCh as a substrate. Error bars represent the standard deviation (SD) of 6 independent experiments. b Staining of DjChE activity in fixed D. japonica using ATCh (i) and BTCh (ii) substrates show activity is localized to the nervous system (* indicates the cephalic ganglion or brain and arrows indicate the ventral nerve cords) without clear spatial discrimination between the two activities. c Inhibition kinetics from 500 μM (circles), 1 mM (squares), and 5 mM (diamonds) iso-OMPA on DjChE activity using 1mM ATCh (black solid line) and BTCh (gray dashed line) as substrates. Activity is reported as the percent of the mean activity in the solvent control samples