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. Author manuscript; available in PMC: 2019 Apr 26.
Published in final edited form as: Immunohorizons. 2019 Apr 1;3(4):133–148. doi: 10.4049/immunohorizons.1900022

FIGURE 10. IFN-γ–producing cells in the spleen and lungs postchallenge.

FIGURE 10

After priming with QIV, C57BL/6 mice were immunized with different formulations, as described in Fig. 7, and the animals were challenged on day 84 with H1N1pdm09 virus. Their spleens and lungs were collected on day 90 to quantify the Ag-specific T cells in these organs. The cells isolated from the spleen (A–E) or the lungs (F–I) were stimulated with either the NP-derived peptide ASNENMETM (NP specific) (A and F), a pool of peptides spanning the influenza virus NP (NP overlap) (B), whole virus H1N1pdm09 (C and G), a pool of peptides spanning the H1N1pdm09 HA (HA overlap) (D and H), or the irrelevant peptide RAHYNIVTF (derived from human papillomavirus E7) (E and I), and the number of IFN-γ–producing cells was measured by ELISPOT. *p , 0.05, **p , 0.01, ***p , 0.001, ****p , 0.0001.