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. Author manuscript; available in PMC: 2019 Apr 26.
Published in final edited form as: Immunohorizons. 2019 Apr 1;3(4):133–148. doi: 10.4049/immunohorizons.1900022

FIGURE 6. Functionality of the vaccine-elicited anti-H1 stalk Abs.

FIGURE 6

After priming with QIV, BALB/c mice were immunized with different formulations, as described in Fig. 2, and the functionality of the elicited anti-H1 stalk Abs collected on day 84 was evaluated in vitro by using an ADCC reporter assay. The specific activation of effector cells against cH6/1N5- (A) or H1N1pdm09 (B)-infected MDCK cells was analyzed by the measurement of luciferase activity. Sera collected on day 84 were also pooled and transferred into naive BALB/c mice. These mice were then challenged with a lethal dose of cH6/1N5 virus (C and D) or H1N1pdm09 virus (E and F), and their weight (C and E) and survival (D and F) were monitored until recovery (16 or 14 d, respectively).