Figure 4.
Immunoblot analysis of SS distribution in the tip and axis regions of maize primary root. A and B, Distribution of SS in soluble and membrane fractions, respectively. Roots from aerobic and 48-h submerged seedlings were separated into tip and axis regions before protein extraction. T0 and Ax0, Tip and axis regions from aerobic seedlings; T48 and Ax48, tips and axes from 48-h submerged seedlings. C and D, Time course of SS accumulation in microsomal fractions of root tips and axes, respectively. Maize seedlings were submerged for indicated duration (in hours). Microsomes were prepared from root tips and axes separately and used for immunoblotting. T0 and Ax0 to T48 and A48, Tips and axes from seedlings submerged for 0 to 48 h.