Figure 5.

Immunoblot showing the differential intracellular localization of SS in sh1 and sus1 mutants. A, Membrane distribution of SS in the root tissue of non-mutant and mutant maize seedlings. B, The distribution of SS in the root soluble fractions of mutant genotypes. Air, Aerobic; S, 48-h submerged; NM, non-mutant; sh and sus, mutants lacking SH1 and SUS1, respectively (though sus1 is known to be a leaky mutant with aberrant transcripts, no signals were detected with the monoclonal antisera used).