Table I.

Binding assay of PTF1 to the wild-type and modified psbD LRP sequences

Strain	Target Sequencea	Phenotype
Wild type	AAAAGAAAAAGTAAGTGGACCTAACCC	LacZ+b
mt-1	CCCCTCCCAAGTAAGTGGACCTAACCC	LacZ+
mt-2	AAAAGAAACCTGCCTGGGACCTAACCC	LacZ+
mt-3	AAAAGAAAAAGTAAGTGGTAGTATGAC	LacZ−b

Yeast strains that carry three (mt-1) or four (others) repeats of target sequences followed by lacZ under the control of PCYC1 in their genome DNA were transformed with the PTF1-AD fusion vector and tested for β-galactosidase activity.

^a Replaced nucleotides are indicated with bold letters. 

^b LacZ+ or LacZ− represents the phenotype of transformants according to the β-galactosidase assay.